Acute myeloid leukemia is associated with retroviral gene transfer to hematopoietic progenitor cells in a rhesus macaque.

نویسندگان

  • Ruth Seggewiss
  • Stefania Pittaluga
  • Rima L Adler
  • F Javier Guenaga
  • Cole Ferguson
  • Ingo H Pilz
  • Byoung Ryu
  • Brian P Sorrentino
  • W Scott Young
  • Robert E Donahue
  • Christof von Kalle
  • Arthur W Nienhuis
  • Cynthia E Dunbar
چکیده

We report, for the first time, a replication-defective retroviral vector-associated neoplasia in a nonhuman primate. Five years after transplantation with CD34+ cells transduced with a retroviral vector expressing enhanced green fluorescent protein (eGFP) and a drug-resistant variant of the dihydrofolate reductase gene (L22Y), a rhesus macaque developed a fatal myeloid sarcoma, a type of acute myeloid leukemia. Tumor cells contained 2 clonal vector insertions. One insertion was found in BCL2-A1, an antiapoptotic gene. This event suggests that currently available retroviral vectors may have long-term side effects, particularly in hematopoietic stem and progenitor cells.

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عنوان ژورنال:
  • Blood

دوره 107 10  شماره 

صفحات  -

تاریخ انتشار 2006